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Updated: Jun 16, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
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Aberrant Cortical Activity in Multiple GCaMP6-Expressing Transgenic Mouse Lines.

Nicholas A Steinmetz1,2, Christina Buetfering3,4, Jerome Lecoq5

  • 1UCL Institute of Neurology, University College London, London, UK.

Eneuro
|September 22, 2017
PubMed

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Summary
This summary is machine-generated.

Some transgenic mouse lines exhibit abnormal brain activity, resembling epilepsy, when using GCaMP6 calcium sensors. Researchers should be aware of these findings when interpreting neuroscience data from these specific mouse models.

Area of Science:

  • Neuroscience
  • Genetics
  • Biotechnology

Background:

  • Transgenic mouse lines are essential for neuroscience research.
  • Genetically encoded calcium sensors like GCaMP6 are widely used to monitor neural activity.
  • Potential artifacts in experimental tools can impact research findings.

Purpose of the Study:

  • To report and characterize aberrant electrical and fluorescence activity in specific transgenic mouse lines.
  • To raise awareness among researchers about potential confounding factors in neuroscience experiments.
  • To investigate the prevalence and characteristics of these events across different laboratories.

Main Methods:

  • Observation of local field potentials and widefield calcium imaging.
  • Analysis of transgenic mouse lines, particularly those with Emx1-Cre and Ai93 transgenes.
Keywords:
CortexGCaMPepilepsytransgenic

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  • Cross-laboratory validation of observed phenomena.

    • Main Results:

    • Aberrant, epileptiform electrical activity and fluorescence events were observed in specific transgenic mouse genotypes.
    • These events occurred at high frequency (>0.1 Hz) and large amplitude (>1.0 mV LFP, >10% df/f imaging).
    • The phenomena were observed across multiple laboratories and in both sexes, though not exclusively in Emx1-Cre/Ai93 mice.

    Conclusions:

    • The observed epileptiform events in GCaMP6-expressing transgenic mice may confound neuroscience research.
    • Researchers using these mouse lines should be vigilant when interpreting neuronal activity and behavioral data.
    • Further investigation into the underlying mechanisms of these aberrant events is warranted.