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Updated: Feb 22, 2026
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
Published on: February 8, 2016
[Which technique should be used in the phenotyping of lymphocytic alveolitis: Immunocytochemistry or flow cytometry]
Mona Mlika1, Rihem Kasmi1, Ines Safra2
1Service d'anatomie pathologique, hôpital Abderrahmam-Mami, Ariana, 2036 Tunisie.
Background:
Diffuse interstitial pneumonias are considered as a group of multiple affections characterized by challenging diagnoses because of the lack of specific clinical signs. Radiologic investigations highlight the diagnoses in most of the cases but bronchoalveolar lavage plays a key role in the diagnostic diagram. We aim to compare the immunocytochemical technique and the flow cytometry in the phenotyping of lymphocytic alveolitis.
Methods:
We described a series of 32 lymphocytic alveolitis, which were analyzed using immunocytochemistry and flow cytometry.
Results:
We found a good reproducibility between the immunocytochemistry performed on smears and cytoblocks (kappa=0.7) and a poor reproducibility between immunocytochemistry and flow cytometry (kappa=0.35).
Conclusion:
Our study emphasized on the poor reproducibility between immunocytochemistry and flow cytometry. Further studies about the reliability of both techniques are needed especially in discordant cases.
