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["Push-pull" perfusion technic in neuroendocrinology].

S Arancibia1

  • 1Laboratoire de Neurobiologie Endocrinologique UA 1197 du CNRS, Université de Montpellier II (USTL).

Annales D'Endocrinologie
|January 1, 1987
PubMed
Summary

Push-pull perfusion (PPP) is a valuable tool for studying neurohormone release in the median eminence (ME). Optimizing technical aspects like cannula diameter and perfusion flow enhances the reliability of PPP in neuroendocrinology research.

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Area of Science:

  • Neuroendocrinology
  • Physiology
  • Neuroscience

Background:

  • The median eminence (ME) is crucial for neurohormone release.
  • Push-pull perfusion (PPP) is a technique used to study neurohormone release.
  • Discrepancies exist in PPP studies, potentially due to technical variations.

Purpose of the Study:

  • To review different push-pull cannula device models used in neuroendocrinology.
  • To identify technical factors influencing PPP results in ME studies.
  • To compare PPP measurements with portal cannula measurements.

Main Methods:

  • Review of push-pull cannula devices for ME neurohormone studies.
  • Analysis of technical parameters: cannula diameter, perfusion flow rate, and implantation timing.
  • Comparison of neurohormone release (SRIF, TRH, CRF) measured by PPP versus portal cannula.

Main Results:

  • Technical variations in PPP (cannula size, flow rate, implantation time) can explain study disagreements.
  • Physiological stimuli elicit a more significant response in perfusate compared to portal blood.
  • PPP allows for in vivo studies of neurohormone release.

Conclusions:

  • Despite limitations in reproducibility and yield, PPP is a major methodological tool for in vivo neuroendocrinology.
  • Optimizing PPP techniques is essential for reliable neurohormone release studies.
  • PPP offers optimal conditions for studying neurohormone dynamics in the median eminence.

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