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Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
Published on: December 29, 2017
Lysosomal tracking with a cationic naphthalimide using multiphoton fluorescence lifetime imaging microscopy
Meng Li1, Haobo Ge, Vincenzo Mirabello
1Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK. t.d.james@bath.ac.uk s.pascu@bath.ac.uk.
Abstract:
A naphthalimide-based chemosensing motif turns ON the fluorescence emission in solution in the presence of aqueous iron(iii) chloride, and maintains this property in living cancer cells. The emission response to Fe(iii) ions occurs simultaneously with a change in pH. The protonation of methyl piperazine-conjugated naphthalimide promotes its lysosomal localisation as assessed by co-localisation tests and fluorescence lifetime imaging microscopy (FLIM) in vitro.

