Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Skin Cancer in the Rural South: Strengthening Dermatologic Training for Frontline Primary Care.

Cureus·2026

Same author

Longer Pembrolizumab Therapy Reduces Mortality in Stage III and IV Melanoma: A TriNetX Study.

Journal of drugs in dermatology : JDD·2026

Same author

Excited Skin Syndrome (Angry Back), What Do We Know About It? A Review of the Literature.

Journal of cosmetic dermatology·2026

Same author

The Effect of Sequential Topical Application of Dermatologic Medications on Absorption: Clinical Considerations.

Skin pharmacology and physiology·2026

Same author

Eyelid dermatitis: Work-up and future diagnostic innovative solutions.

Progress in retinal and eye research·2025

Same author

Neurocosmetics are cosmetics, which mean that they could have effects only on skin.

Clinics in dermatology·2025

Same journal

Bidirectional association between immunobullous diseases and obsessive-compulsive disorder including excoriation disorder.

Archives of dermatological research·2026

Same journal

Diagnosis of immunobullous diseases in underrepresented groups: an all of us database study.

Archives of dermatological research·2026

Same journal

Increased Risk of Melanoma and Basal Cell Carcinoma in Patients with Sjogren's Syndrome: A Nested Case-Control Study.

Archives of dermatological research·2026

Same journal

Vitiligo and Risk of Gastrointestinal Disorders in the United States: A Case-Control Study.

Archives of dermatological research·2026

Same journal

Increased Risk of Sensorineural Hearing Loss in Patients with Vitiligo: A Nested Case-Control Study.

Archives of dermatological research·2026

Same journal

Transgender Individuals' Perspectives on Teledermatology-Based Acne Research.

Archives of dermatological research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 22, 2026

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

Dermal-epidermal separation methods: research implications.

Ying Zou^1,2, Howard I Maibach³

¹Skin and Cosmetic Research Department, Shanghai Skin Disease Hospital, 1278 Baode Road, Shanghai, 200443, People's Republic of China.

Archives of Dermatological Research

|September 23, 2017

Summary

This summary is machine-generated.

Choosing the right dermal-epidermal separation method is crucial for research. Each technique (chemical, enzyme, heat, mechanical) has unique pros and cons, with no single superior option for all applications.

Keywords:

Chemical Dermis Enzymes Epidermis Heat Mechanical Separation

More Related Videos

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Published on: August 25, 2018

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Related Experiment Videos

Last Updated: Feb 22, 2026

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Published on: August 25, 2018

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Area of Science:

Dermatology
Pharmacology
Toxicology
Biology

Background:

Dermal-epidermal separation is a fundamental technique in biological sciences.
Selecting an optimal separation method is critical for accurate research outcomes.

Purpose of the Study:

To review and compare various dermal-epidermal separation techniques.
To guide researchers in choosing the most appropriate method for their specific needs.

Main Methods:

Systematic literature review of studies on epidermal separation.
Databases searched: PubMed, Embase, and Web of Science.
Comparison of chemical, enzyme, heat, and mechanical separation methods.

Main Results:

Heat separation is simple but causes thermal damage.
Chemical reagents are effective but disrupt electrolyte balance.
Enzymatic methods offer complete separation but degrade components.
Mechanical methods preserve integrity but require large samples and risk cross-contamination.

Conclusions:

No single dermal-epidermal separation method is universally superior.
The choice of method depends on the specific research question and requirements.
Careful consideration of advantages and disadvantages is necessary for optimal technique selection.