Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rate of IPG Placement for Sacral Neuromodulation in Patients With CNS Pathology.

Urogynecology (Philadelphia, Pa.)·2026

Same author

Targeted knockout of a host peroxisomal peptidase confers field resistance to maize lethal necrosis.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Target trial emulation without matching: a more efficient approach for evaluating vaccine effectiveness using observational data.

Epidemiology (Cambridge, Mass.)·2026

Same author

Does Sacral Colpopexy Increase Constipation Compared With Vaginal Prolapse Surgery?

Urogynecology (Philadelphia, Pa.)·2026

Same author

Transitory Schwann Cell Precursor and hybrid states underpin melanoma therapy resistance and metastasis.

bioRxiv : the preprint server for biology·2025

Same author

Light-chain split luciferase assay implicates pathological NOTCH3 thiol reactivity in inherited cerebral small vessel disease.

The Journal of biological chemistry·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 22, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Agrobacterium-Mediated Sorghum Transformation.

Emily Wu¹, Zuo-Yu Zhao²

¹DuPont Pioneer, Johnston, IA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 23, 2017

Summary

This summary is machine-generated.

This study presents an optimized Agrobacterium-mediated sorghum transformation protocol. The developed method achieves high transformation frequencies, exceeding 10% and 33% with different Agrobacterium strains.

Keywords:

Agrobacterium Plant transformation Selection markers Sorghum Super-binary vector Transformation Transgenic plants Transgenic sorghum

More Related Videos

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Related Experiment Videos

Last Updated: Feb 22, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Area of Science:

Plant Science
Genetics
Biotechnology

Background:

Agrobacterium-mediated transformation is a key tool for crop genome modification.
Efficient transformation protocols are crucial for advancing crop breeding and genetic research.

Purpose of the Study:

To describe an optimized protocol for Agrobacterium-mediated transformation of sorghum.
To evaluate the efficiency of the protocol using different Agrobacterium strains and selection markers.

Main Methods:

Development and optimization of a sorghum transformation protocol.
Utilizing Agrobacterium strains LBA4404 and AGL1.
Employing moPAT and PMI as selection marker genes.

Main Results:

Achieved transformation frequencies over 10% with Agrobacterium strain LBA4404.
Achieved transformation frequencies over 33% with Agrobacterium strain AGL1.
Demonstrated the efficacy of both moPAT and PMI selection marker genes.

Conclusions:

The optimized protocol significantly enhances sorghum transformation efficiency.
The protocol is robust and adaptable to different Agrobacterium strains and selection markers.
This advancement facilitates genetic improvement of sorghum.