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Updated: Feb 22, 2026
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Electrochemiluminescence resonance energy transfer system between GNRs and Ru(bpy)32+: Application in magnetic
Hong Ke1, Haifeng Sha1, Yinfang Wang1
1The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, Shanghai Normal University, Shanghai 200234, People's Republic of China.
Abstract:
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest in the biomedical analysis. This paper proposed a new methodology for ultrasensitive and facile detection of Alzheimer's disease marker β-amyloid (Aβ) by fabricating a sandwich-type ECL sensing platform. Herein, electrochemiluminescence resonance energy transfer (ECL-RET) was employed to determine Aβ concentration, which can be attributed to the quenching effect from RET between Ru(bpy)32+ and gold nanorods (GNRs) acting as ECL-RET electron donor and acceptor, respectively. In this protocol, mesoporous carbon nanospheres were adopted to immobilize ECL reactant Ru(bpy)32+ and antibody via nafion to acquire the RET donor nanocomposites (MOCs/nafion/Ru(bpy)32+/antibody), which were tightly interconnected with epoxy group functionalized Fe3O4 nanoparticles. It is of vital importance that GNRs with exquisite rod shape were synthesized and exhibited a typical absorption peak at 650nm to quench ECL signal of Ru(bpy)32+ effectively. In addition, the ECL emission decreased linearly with the logarithm of Aβ concentration in a wide linear range from 1.0 × 10-5 to 100ng/mL with a detection limit of 4.2 × 10-6ng/mL. Furthermore, distinctive and desirable properties were verified to declare the promise for being applicable to analyze the Aβ content in real Alzheimer's cerebrospinal fluid samples with satisfactory results.
