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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
Published on: April 19, 2018
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SOX7 Is Required for Muscle Satellite Cell Development and Maintenance
Rashida F Rajgara1, Neena Lala-Tabbert2, François Marchildon3
1Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Graduate Program in Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
Stem Cell Reports
|September 26, 2017
Summary
SOX7 is crucial for maintaining skeletal muscle stem cells (satellite cells) and promoting their survival and development. Loss of SOX7 impairs muscle regeneration and survival, though it is not essential for myogenesis itself.
Area of Science:
- Muscle stem cell biology
- Skeletal muscle regeneration
- Transcription factor function
Background:
- Satellite cells are essential for skeletal muscle repair and regeneration.
- SOX7, a transcription factor, is present in quiescent satellite cells.
- Understanding SOX7's role is key to muscle repair mechanisms.
Purpose of the Study:
- To investigate the function of SOX7 in skeletal muscle stem cells.
- To determine SOX7's role in myogenesis and satellite cell survival.
- To elucidate the impact of SOX7 deficiency on muscle regeneration.
Main Methods:
- Knockdown of Sox7 in embryonic stem cells and primary myoblasts.
- Generation of a conditional knockout mouse for Sox7 in PAX3+ cells.
- Assessment of satellite cell population, myoblast differentiation, fusion, and apoptosis.
Main Results:
- Loss of SOX7 reduced Pax3 and Pax7 expression in embryonic stem cells.
- Conditional Sox7 knockdown decreased satellite cell numbers and impaired muscle regeneration.
- SOX7 deficiency led to impaired myoblast fusion and increased apoptosis.
Conclusions:
- SOX7 is dispensable for myogenesis (muscle formation).
- SOX7 is essential for satellite cell development and survival.
- SOX7 plays a critical role in maintaining skeletal muscle stem cell populations and promoting regeneration.
Keywords:
PAX3PAX7SOX7conditional knockoutembryonic stem cellmuscle regenerationmuscle satellite cellsmyogenesisMore Related Videos
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