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miRNA biogenesis-associated RNase III nucleases Drosha and Dicer are upregulated in colorectal adenocarcinoma

Shin Kim1,2, Mei Ling Song3, Hyeonji Min1

  • 1Department of Immunology, School of Medicine, Keimyung University, Dalseo, Daegu 42601, Republic of Korea.

Oncology Letters
|September 26, 2017
PubMed

Insights

Drosha and Dicer, key microRNA regulators, are significantly upregulated in colorectal cancer (CRC) tissues. Their expression levels in CRC did not correlate with clinical features or each other.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Drosha and Dicer are crucial enzymes in microRNA (miRNA) biogenesis.
  • Aberrant regulation of these enzymes is implicated in colorectal cancer (CRC) development.
  • Understanding their role in CRC pathogenesis is vital for potential therapeutic strategies.

Purpose of the Study:

  • To investigate the messenger RNA (mRNA) expression levels of Drosha and Dicer in colorectal cancer (CRC) specimens.
  • To determine the association between Drosha and Dicer mRNA expression and clinical features in CRC patients.
  • To explore the relationship between Drosha and Dicer mRNA expression levels in CRC.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) was used to measure Drosha and Dicer mRNA expression.
  • The study analyzed 77 colorectal cancer (CRC) tissues and adjacent non-neoplastic tissues.
  • Clinical data including sex, age, TNM stage, BMI, and CEA levels were collected.

Main Results:

  • Drosha and Dicer mRNA expression levels were significantly upregulated in colorectal cancer (CRC) tissues compared to non-neoplastic tissues.
  • No significant association was found between Drosha or Dicer mRNA expression levels and clinical parameters (sex, age, TNM stage, BMI, CEA).
  • There was no significant correlation between the expression levels of Drosha and Dicer mRNA in CRC specimens.

Conclusions:

  • The study demonstrates significant upregulation of Drosha and Dicer mRNA in colorectal cancer (CRC).
  • These findings suggest that Drosha and Dicer play a role in the pathobiology of colorectal carcinogenesis.
  • Further research is warranted to elucidate the precise mechanisms and clinical implications of Drosha and Dicer dysregulation in CRC.
Keywords:
DicerDroshacolorectal adenocarcinomamicroRNA biogenesis

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