Processable enzyme-hybrid conductive polymer composites for electrochemical biosensing

Area of Science:

• Materials Science
• Electrochemistry
• Biotechnology

Background:

• Electrochemical biosensors are crucial for diagnostics.
• Conducting polymers offer unique properties for biosensor development.
• Efficient immobilization of enzymes and nanoparticles is key for biosensor performance.

Purpose of the Study:

• To develop a facile fabrication method for electrochemical biosensors.
• To create a novel biohybrid conducting polymer using glucose oxidase (GOx) and poly (3, 4-ethylenedioxythiophene) (PEDOT).
• To evaluate the performance of the developed glucose biosensor.

Main Methods:

• Template-assisted chemical polymerization to form PEDOT microspheres (PEDOT-MSs).
• In-situ deposition of platinum nanoparticles (PtNPs) onto PEDOT-MSs.
• Electrostatic immobilization of glucose oxidase (GOx) to create GOx-PtNPs-PEDOT-MSs.
• Characterization using SEM, EDS, FTIR, zeta potential, and electrochemical measurements.

Main Results:

• The GOx-PtNPs-PEDOT-MS biosensor showed a linear glucose response from 0.1-10mM (R² = 0.9855).
• Achieved a high sensitivity of 116.25µAmM⁻¹cm⁻² and a low limit of detection of 1.55µM.
• Demonstrated significantly higher sensitivity (2.7 times) compared to existing PEDOT-based glucose biosensors.
• Exhibited good storage stability, retaining 97% sensitivity after 12 days.

Conclusions:

• The novel bio-hybrid conducting polymer offers a convenient material for electrochemical biosensors.
• The developed material combines micro-structured morphology, processability, and intrinsic biocatalytic activity.
• This approach shows great potential for applications in printed bioelectronics and sensors.