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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Feb 22, 2026

Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy

Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy

Published on: March 25, 2022

Sheathless Microflow Cytometry Using Viscoelastic Fluids.

Mohammad Asghari¹, Murat Serhatlioglu¹, Bülend Ortaç¹

¹UNAM - National Nanotechnology Research Center, Institute of Materials Science and Nanotechnology, Bilkent University, 06800, Ankara, Turkey.

Scientific Reports

|September 29, 2017

Summary

This summary is machine-generated.

This study introduces a sheathless microflow cytometer using viscoelastic focusing for particle analysis. This innovative device achieves high throughput and precision, simplifying particle characterization in microfluidic systems.

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Last Updated: Feb 22, 2026

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Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

Published on: April 25, 2013

Area of Science:

Microfluidics
Biotechnology
Analytical Chemistry

Background:

Microflow cytometry is crucial for particle characterization in solutions.
Traditional methods often require complex sheath flow systems.
Viscoelastic focusing offers a promising alternative for particle manipulation.

Purpose of the Study:

To develop and demonstrate a novel sheathless microflow cytometer.
To utilize viscoelastic focusing for 3D single-line particle alignment.
To assess the performance of this system for microparticle detection.

Main Methods:

A straight capillary-based microflow cytometer was designed.
Viscoelastic focusing was employed to align microparticles without sheath flow.
Optical detection was achieved using fiber-coupled light sources and photodetectors.
Rheological properties of solutions were measured to analyze focusing performance.

Main Results:

Achieved 3D single-line focusing of microparticles in a straight capillary.
Demonstrated sheathless microflow cytometry with polyethylene oxide (PEO) and hyaluronic acid (HA) solutions.
Reported a throughput of 780 particles/s and 5.8% CV for forward scatter with HA-based focusing.
Showcased performance comparable to state-of-the-art flow cytometers.

Conclusions:

Viscoelastic focusing enables effective sheathless microflow cytometry.
The developed system offers simplicity and high performance.
This technology has the potential to expand the applications of microflow cytometry.