Related Experiment Video
Updated: Feb 22, 2026
Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
Published on: November 1, 2024
Identification of Crocus sativus and its Adulterants from Chinese Markets by using DNA Barcoding Technique
Wei-Juan Huang1, Fei-Fei Li1, Yu-Jing Liu1
1Department of Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, PR China.
Background:
Saffron (Crocus sativus L.) is a common but very expensive herbal medicine. As an important traditional medicine, it has an outstanding effect in treating irregular and painful menstruation. Recently, the over-demand tendency of saffron results in an unusual phenomenon in the medicinal markets. Adulterants and saffron-like substitutes are intentionally mixed into medicinal markets and pharmacies or online stores, affecting drug safety and food quality.
Objectives:
Our study aimed to identify saffron from its adulterants via DNA barcoding.
Materials And Methods:
Samples (13 saffron + 4 others containing Carthamus tinctorius or Chrysanthemum x morifolium) obtained from 12 different provinces of China. Through DNA barcoding, samples were compared using three candidate markers, trnH-psbA, rbcL-a and ITS2.
Results:
trnH-psbA and rbcL-a were capable of distinguishing different accessions. ITS2 could identify samples even at intra-specific level. According to these three barcodes, four samples were identified saffron-like substitutes.
Conclusions:
The adulterant rate in Chinese markets reaches as high as 33.33% that may cause health risks and further may reduce saffron efficacy once is being used as herbal remedy. In order to make a distinction between C. sativus with other genera as adulterants, DNA barcoding is suggested.
