Jove
Visualize
Contact Us

Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

5.3K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
5.3K
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

4.1K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases.

Molecular cell·2012
Same author

Regulation of cellular levels of Sprouty2 protein by prolyl hydroxylase domain and von Hippel-Lindau proteins.

The Journal of biological chemistry·2011
Same author

Stable heteroleptic complexes of divalent lanthanides with bulky pyrazolylborate ligands--iodides, hydrocarbyls and triethylborohydrides.

Dalton transactions (Cambridge, England : 2003)·2010
Same author

Structural and functional characterization of the monomeric U-box domain from E4B.

Biochemistry·2009
Same author

Comparison of covalency in the complexes of trivalent actinide and lanthanide cations.

Journal of the American Chemical Society·2002
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 22, 2026

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

6.0K

Crystal Nucleation Using Surface-Energy-Modified Glass Substrates.

Kyle A Nordquist1, Kevin M Schaab1, Jierui Sha1

  • 1DeNovX, 3440 South Dearborn Street, Lab 204 S, Chicago, Illinois 60616, United States.

Crystal Growth & Design
|October 3, 2017
PubMed
Summary
This summary is machine-generated.

Surface energy modifications on glass accelerate crystallization for active pharmaceutical ingredients (APIs) and proteins. This technique reduces crystallization onset times by up to 52% without altering standard workflows.

More Related Videos

Production of Synthetic Nuclear Melt Glass
04:36

Production of Synthetic Nuclear Melt Glass

Published on: January 4, 2016

9.9K
Fabricating van der Waals Heterostructures with Precise Rotational Alignment
09:25

Fabricating van der Waals Heterostructures with Precise Rotational Alignment

Published on: July 5, 2019

10.2K

Related Experiment Videos

Last Updated: Feb 22, 2026

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
12:29

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Published on: March 13, 2021

6.0K
Production of Synthetic Nuclear Melt Glass
04:36

Production of Synthetic Nuclear Melt Glass

Published on: January 4, 2016

9.9K
Fabricating van der Waals Heterostructures with Precise Rotational Alignment
09:25

Fabricating van der Waals Heterostructures with Precise Rotational Alignment

Published on: July 5, 2019

10.2K

Area of Science:

  • Materials Science
  • Crystallography
  • Pharmaceutical Science

Background:

  • Controlling nucleation is crucial for successful crystallization of active pharmaceutical ingredients (APIs) and proteins.
  • Existing methods for inducing nucleation often require significant changes to experimental setups or workflows.

Purpose of the Study:

  • To investigate the impact of systematic surface energy modifications on glass substrates for inducing and improving crystallization outcomes.
  • To assess the broad applicability of surface energy modifications across various active pharmaceutical ingredients (APIs), proteins, solvents, and crystallization techniques.

Main Methods:

  • Systematic examination of various active pharmaceutical ingredients (APIs), proteins, organic solvents, aqueous media, surface energy motifs, and crystallization methods.
  • Implementation of both flat and convex surface energy modifications on glass substrates.
  • Utilized bulk cooling and microdomain cooling techniques for small molecules and vapor diffusion for proteins.
  • Engineered nucleation site arrays onto common crystallization form factors like slides, vials, and high-throughput screening plates.

Main Results:

  • Demonstrated an average reduction in crystallization onset times of 52(4)% for acetylsalicylic acid using flat and convex surface energy modifications.
  • Achieved a 32(2)% reduction in crystallization onset times for thaumatin and bovine pancreatic trypsin via vapor diffusion experiments.
  • Successfully engineered nucleation site arrays on various standard crystallization vessels.

Conclusions:

  • Systematic surface energy modifications are effective in inducing nucleation and accelerating crystallization for both small molecule APIs and proteins.
  • This approach offers a versatile tool to enhance crystallization screening without necessitating major changes to existing laboratory workflows or instrumentation.
  • Engineered nucleation sites provide a controllable variable for optimizing crystallization outcomes.