Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

FN-silk membrane enables alveologenesis processes and self-organization of the H441 epithelial cell line into native-like alveolar morphology.

Scientific reports·2026

Same author

A Molecular "Thermometer" for Measuring Effective Non-Local Exchange.

Journal of computational chemistry·2026

Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026

Same author

Microbial Electrosynthesis Reshapes Energy Metabolism and Physiology in Clostridium ljungdahlii.

Microbial biotechnology·2026

Same author

Automating the Integration of Longitudinal Clinical Trial Data Using REDCap.

Studies in health technology and informatics·2026

Same author

From Excel to Automation: The Transplant Mapper for Interoperable Transplant Data Management.

Studies in health technology and informatics·2026

Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026

Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026

Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026

Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026

Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026

Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 22, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.

Sara Abrahamsson¹, Hans Blom², Ana Agostinho³

¹Lulu and Anthony Wang Laboratory for Neural Circuits and Behavior, The Rockefeller University New York, NY 10021, USA.

Biomedical Optics Express

|October 3, 2017

Summary

This summary is machine-generated.

We combined structured illumination microscopy (SIM) with multifocus microscopy (MFM) to significantly speed up 3D super-resolution biological imaging. This novel approach enhances volumetric acquisition speed by tenfold for faster biological specimen analysis.

Keywords:

(100.6640) Superresolution (170.0110) Imaging systems (170.2520) Fluorescence microscopy (180.0180) Microscopy (180.2520) Fluorescence microscopy (180.6900) Three-dimensional microscopy

More Related Videos

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Related Experiment Videos

Last Updated: Feb 22, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Area of Science:

Biophysics
Microscopy
Optical Imaging

Background:

Super-resolution microscopy techniques like SIM offer enhanced resolution beyond the diffraction limit.
Traditional 3D super-resolution imaging often suffers from slow volumetric acquisition speeds, limiting dynamic biological studies.
Multifocus microscopy (MFM) enables simultaneous 3D imaging but has not been integrated with super-resolution methods.

Purpose of the Study:

To introduce and validate a synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM).
To address the bottleneck of slow volumetric acquisition in super-resolution biological imaging.
To demonstrate improved imaging speed and performance on biological specimens.

Main Methods:

Integration of multifocus diffractive optics into the SIM acquisition sequence.
Development of a combined SIM-MFM imaging modality.
Validation using imaging of biological specimens.

Main Results:

Achieved simultaneous wide-field 3D imaging capability within the SIM acquisition.
Demonstrated an order of magnitude improvement in volumetric acquisition speed.
Successfully imaged biological specimens with the enhanced modality.

Conclusions:

The synergistic SIM-MFM approach significantly accelerates volumetric super-resolution imaging.
This technique overcomes limitations in acquisition speed for 3D biological imaging.
The combined modality shows promise for advancing dynamic studies in cell biology and beyond.