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Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
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Hyperspectral imaging with laser-scanning sum-frequency generation microscopy.

Adam Hanninen1, Ming Wai Shu2, Eric O Potma2,3

  • 1Department of Astronomy and Physics, University of California, Irvine, CA 92697, USA.

Biomedical Optics Express
|October 3, 2017
PubMed
Summary
This summary is machine-generated.

Sum-frequency generation (SFG) microscopy, a chemical imaging tool, is now faster and more accessible. Minor microscope modifications enable rapid, high-resolution SFG imaging of biological samples.

Keywords:
(110.3080) Infrared imaging(170.0180) Microscopy(180.4315) Nonlinear microscopy(190.4223) Nonlinear wave mixing

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Area of Science:

  • Nonlinear Optics
  • Biophotonics
  • Chemical Imaging

Background:

  • Sum-frequency generation (SFG) microscopy offers chemical selectivity for biological samples.
  • Integrating mid-infrared light into laser-scanning microscopes has limited SFG microscopy's routine use.
  • Previous point-scanning SFG microscopes had slow image acquisition rates.

Purpose of the Study:

  • To adapt conventional laser-scanning microscopes for SFG imaging.
  • To improve image acquisition speed and spatial resolution for SFG microscopy.
  • To demonstrate the capabilities of SFG microscopy in biological sample analysis.

Main Methods:

  • Minor modifications to a standard laser-scanning nonlinear optical (NLO) microscope.
  • Integration of mid-infrared excitation light for SFG.
  • Implementation of hyperspectral imaging and multimodal imaging (SFG, SHG, CARS).

Main Results:

  • Achieved vibrationally sensitive SFG imaging of biological samples with sub-micrometer resolution.
  • Reached image acquisition rates of 1 frame/s, significantly faster than previous methods.
  • Demonstrated hyperspectral SFG imaging and multimodal imaging capabilities.

Conclusions:

  • SFG microscopy can be readily integrated into existing laser-scanning NLO microscopes.
  • The developed method dramatically increases SFG imaging speed and maintains high resolution.
  • SFG microscopy is now a practical and valuable tool comparable to other NLO imaging techniques for biological studies.