Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The fluorescence-activating and absorption-shifting tag (FAST), a versatile protein marker for live plant cell imaging.

The Plant journal : for cell and molecular biology·2026
Same author

Chemogenetic Modulation of Luciferase Emission Color for Imaging and Sensing.

ACS sensors·2026
Same author

Interplay between high-energy quenching and state transitions in Chlamydomonas reinhardtii: a single-cell approach.

The New phytologist·2026
Same author

General data protection regulation: an algorithmic proposal for forensic photography.

International journal of legal medicine·2026
Same author

Versatile luminescence macroscope with dynamic illumination for photoactive systems.

Optics express·2025
Same author

Unequal mitochondrial segregation promotes asymmetric fates during neurogenesis.

Nature communications·2025

Related Experiment Video

Updated: Feb 21, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.1K

Dynamic multicolor protein labeling in living cells.

Chenge Li1,2, Marie-Aude Plamont1,2, Hanna L Sladitschek3

  • 1École Normale Supérieure , PSL Research University , UPMC Univ Paris 06 , CNRS , Département de Chimie , PASTEUR , 24 rue Lhomond , 75005 Paris , France.

Chemical Science
|October 4, 2017
PubMed
Summary

Researchers developed new dyes to change the color of the Yellow Fluorescence-Activating and absorption-Shifting Tag (FAST) from green-yellow to red. This allows for multicolor live-cell imaging and dynamic color switching, advancing multiplexed imaging techniques.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

6.1K

Related Experiment Videos

Last Updated: Feb 21, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

6.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • The Yellow Fluorescence-Activating and absorption-Shifting Tag (FAST) is a 14 kDa protein tag that forms a bright green-yellow fluorescent complex with the dye HMBR.
  • Current limitations exist in multicolor imaging due to spectral overlap and the need for distinct fluorescent probes.

Purpose of the Study:

  • To develop a suite of fluorogens that enable tunable fluorescence emission colors for the FAST protein tag.
  • To demonstrate the utility of these new fluorogens for multicolor live-cell imaging and dynamic color switching applications.

Main Methods:

  • Synthesis and characterization of novel fluorogenic dyes designed to interact with the FAST protein.
  • Live-cell imaging experiments utilizing FAST-tagged proteins and the developed fluorogens.
  • Two-color cross-correlation analysis to assess multiplexing capabilities.

Main Results:

  • A collection of fluorogens was created, enabling the FAST tag's fluorescence color to be tuned from green-yellow to orange and red.
  • Demonstrated multicolor imaging of FAST-tagged proteins in live cells.
  • Showcased dynamic color switching capabilities due to FAST's reversible labeling.

Conclusions:

  • The developed fluorogens significantly expand the spectral range of FAST, enabling multicolor imaging and overcoming spectral crowding.
  • The reversible labeling and dynamic color switching properties of FAST with these new fluorogens open new avenues for advanced multiplexed imaging in live cells.