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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Photoluminescence: Fluorescence and Phosphorescence01:23

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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
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Fluorescence and Phosphorescence: Instrumentation01:25

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Versatile luminescence macroscope with dynamic illumination for photoactive systems.

Ian Coghill, Alienor Lahlou, Andrea Lodetti

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    Researchers can now perform advanced luminescence imaging with a new, accessible system. This versatile fluorescence imaging setup includes build instructions and open-source software, lowering barriers for biological and material science studies.

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    Area of Science:

    • Biophysics
    • Materials Science
    • Optical Imaging

    Background:

    • Luminescence imaging is crucial for biological and material studies, especially with advanced temporal dynamics.
    • Implementing advanced luminescence imaging often requires specialized, custom-built instrumentation.
    • Lack of expertise in optics, electronics, and software presents a significant barrier for researchers.

    Purpose of the Study:

    • To develop a versatile macroscopic fluorescence imaging system.
    • To provide detailed build instructions and open-source software for system replication.
    • To enable researchers with minimal experience to perform advanced luminescence imaging protocols.

    Main Methods:

    • Construction of a versatile macroscopic fluorescence imaging system.
    • Development of accompanying open-source software.
    • Demonstration of system utility across diverse applications.

    Main Results:

    • The developed system supports a wide range of advanced luminescence imaging protocols.
    • Detailed build instructions and open-source software facilitate system replication.
    • Successful applications demonstrated in plants, fluorescent proteins, and optoelectronic devices.

    Conclusions:

    • The presented imaging system significantly lowers the barrier to entry for advanced luminescence imaging.
    • The open-source nature and detailed instructions promote accessibility and reproducibility.
    • The system's versatility makes it valuable for diverse research fields in biology and materials science.