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Urinary alanine aminopeptidase assay improved as result of multivariate response-surface analysis
C Flandrois1, C Lahet, D Feldmann
1Laboratoire d'Enzymologie (Monique Mathieu), Hôpital Debrousse, Lyon, France.
Clinical Chemistry
|May 1, 1988
Abstract:
Optimization of determination of alanine aminopeptidase in urine by univariate study led to a method involving pretreatment of urine with Sephadex G50. Re-examination of the optimization by multivariate study led us to recommend higher optimal concentrations: 5.8 mmol/L for the substrate and 300 mmol/L for the Tris buffer. Under these new conditions, pretreatment of urine was no longer necessary and the assay could be completely automated.