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Updated: Feb 21, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Analysis of Heterocyclic Amines in Meat by the Quick, Easy, Cheap, Effective, Rugged, and Safe Method Coupled with
Han-Yin Hsiao1, Bing-Huei Chen1, Tsai-Hua Kao1
1Department of Food Science, Fu Jen University , Taipei, Taiwan 242.
Abstract:
The traditional way to analyze heterocyclic amines (HAs) is time-consuming and uses large amounts of solvents. The objective of this study is to develop a quick and simultaneous analysis method for multiple types of HAs contained in meat products. Results showed that 20 HAs and 1 internal standard (4,7,8-TriMeIQx) can be separated within 30 min using an Inspire C18 column and a gradient solvent system containing 10 mM ammonium acetate (pH 2.9) and acetonitrile. This process resulted in a high degree of separation. Using acetonitrile with 1% acetic acid as an extraction solvent, followed by primary and secondary amine, MgSO4, and C18EC as purified reagent, is highly suitable for extracting HAs using the quick, easy, cheap, effective, rugged, and safe method (QuEChERS). Tandem mass spectrometry with selected reaction monitoring mode were used for analysis, which indicated reasonable recovery (58.9-117.4%) for all 20 types of HAs along with limits of detection and quantification in the range of 0.003-0.05 and 0.01-0.05 ng/g, respectively.
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