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Updated: Feb 21, 2026

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
Published on: January 7, 2019
Integrating microalgae tertiary treatment into activated sludge systems for energy and nutrients recovery from
Dulce Maria Arias1, Maria Solé-Bundó1, Marianna Garfí1
1GEMMA - Group of Environmental Engineering and Microbiology, Department of Civil and Environmental Engineering, Universitat Politècnica de Catalunya·BarcelonaTech, c/ Jordi Girona 1-3, Building D1, E-08034 Barcelona, Spain.
Abstract:
In this study, microalgae digestate and secondary effluent were used to grow microalgae in a tertiary wastewater treatment, and then, the biomass was co-digested for biogas generation. A 30L closed-photobioreactor was used for microalgae cultivation. The biomass, mainly composed by Scenedesmus sp., reached and maintained a concentration of 1.1gTSS/L during 30days. A complete removal of N-NH4+ and P-PO43- and high nitrates and organic matter removals were achieved (58% N-NO3- and 70% COD) with 8d of HRT. The potential biogas production of the cultivated microalgae was determined in batch tests. To improve their biodegradability, a novel method combining their co-digestion with activated sludge after a simultaneous autohydrolysis co-pretreatment was evaluated. After the co-pretreatment, the methane yield increased by 130%. Thus, integrating microalgae tertiary treatment into activated sludge systems is a promising and feasible solution to recover energy and nutrients from waste, improving wastewater treatment plants sustainability.
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