Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

13.5K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.5K
Embryonic Stem Cells00:57

Embryonic Stem Cells

5.5K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.5K
Embryonic Stem Cells00:58

Embryonic Stem Cells

33.0K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
33.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic benchmarking of CUT&Tag improves the reliability and reproducibility of chromatin analysis.

Cell reports methods·2026
Same author

MYB builds leukemic enhancers.

Blood·2026
Same author

Results from a prospective registry of <sup>18</sup>F-Fluciclovine PET/CT use in prostate cancer management: a cautionary lesson for implementation of PSMA PET.

American journal of nuclear medicine and molecular imaging·2026
Same author

CHIMERA-DDR: A Machine Learning Framework for Classifying Heterogeneous Mismatch-Repair and Homologous-Recombination Deficiency Patterns in Prostate Cancer.

bioRxiv : the preprint server for biology·2025
Same author

Drug Targets in Prostate Cancer: An Appetite for KLK2-Mediated Destruction.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same author

Mindfulness vs. sleep education during autologous hematopoietic cell transplantation for multiple myeloma: Feasibility of a randomized controlled pilot study.

Contemporary clinical trials communications·2025

Related Experiment Video

Updated: Feb 21, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.7K

Nanog Expression in Embryonic Stem Cells - An Ideal Model System to Dissect Enhancer Function.

Steven Blinka1,2, Sridhar Rao1,2,3

  • 1Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|October 5, 2017
PubMed
Summary

Embryonic stem cells (ESCs) use transcriptional enhancers to control pluripotency. The Nanog locus offers a unique model to study how these enhancers regulate gene expression and cell fate decisions.

Keywords:
eRNAsembryonic stem cellshigher order chromosome structureloopingsuper-enhancerstranscriptional regulation

More Related Videos

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K
Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

363

Related Experiment Videos

Last Updated: Feb 21, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

8.7K
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K
Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

363

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Embryonic stem cells (ESCs) possess pluripotency, the ability to differentiate into any cell type.
  • Pluripotency is regulated by transcription factors (TFs) binding to cis-regulatory elements (CREs).
  • Distal CREs, like enhancers, play a crucial role in modulating gene expression and cell fate.

Purpose of the Study:

  • To review recent advances in understanding transcriptional enhancers.
  • To focus on studies utilizing ESCs as a model system.
  • To highlight the Nanog locus as an informative model for studying enhancer function.

Main Methods:

  • Review of recent scientific literature on transcriptional enhancers in ESCs.
  • Analysis of the Nanog locus for insights into gene regulation.
  • Examination of studies addressing enhancer-gene interactions and cell variability.

Main Results:

  • Transcriptional enhancers are key regulators of pluripotency in ESCs.
  • The Nanog locus provides a unique genomic context for studying enhancer-gene co-regulation.
  • Multiple enhancers near the Nanog gene allow investigation of additive vs. synergistic functions and gene specificity.

Conclusions:

  • ESC pluripotency relies heavily on transcriptional regulation by enhancers.
  • The Nanog locus is a powerful model for dissecting complex regulatory mechanisms.
  • Further research at Nanog can elucidate enhancer function, gene specificity, and cell-to-cell expression variability.