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Updated: Feb 21, 2026
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Published on: January 22, 2014
A cerebrospinal fluid microRNA signature as biomarker for glioblastoma
Johnny C Akers1, Wei Hua2, Hongying Li3
1Center for Theoretical and Applied Neuro-Oncology, University of California, San Diego, CA, USA.
Purpose:
To develop a cerebrospinal fluid (CSF) miRNA diagnostic biomarker for glioblastoma.
Experimental Design:
Glioblastoma tissue and matched CSF from the same patient (obtained prior to tumor manipulation) were profiled by TaqMan OpenArray® Human MicroRNA Panel. CSF miRNA profiles from glioblastoma patients and controls were created from three discovery cohorts and confirmed in two validation cohorts.
Results:
miRNA profiles from clinical CSF correlated with those found in glioblastoma tissues. Comparison of CSF miRNA profiles between glioblastoma patients and non-brain tumor patients yielded a tumor "signature" consisting of nine miRNAs. The "signature" correlated with glioblastoma tumor volume (p=0.008). When prospectively applied to cisternal CSF, the sensitivity and specificity of the 'signature' for glioblastoma detection were 67% and 80%, respectively. For lumbar CSF, the sensitivity and specificity of the signature were 28% and 95%, respectively. Comparable results were obtained from analyses of CSF extracellular vesicles (EVs) and crude CSF.
Conclusion:
We report a CSF miRNA signature as a "liquid biopsy" diagnostic platform for glioblastoma.
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