Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia

MRI provides a sensitive and specific imaging tool to detect acute ischemic stroke by means of a reduced diffusion coefficient of brain water. In a rat model of ischemic stroke, differences in quantitative T₁ and T₂ MRI relaxation times (qT₁ and qT₂) between the ischemic lesion (delineated by low diffusion) and the contralateral non-ischemic hemisphere increase with time from stroke onset. The time dependency of MRI relaxation time differences is heuristically described by a linear function and thus provides a simple estimate of stroke onset time. Additionally, the volumes of abnormal qT₁ and qT₂ within the ischemic lesion increase linearly with time providing a complementary method for stroke timing. A (semi)automated computer routine based on the quantified diffusion coefficient is presented to delineate acute ischemic stroke tissue in rat ischemia. This routine also determines hemispheric differences in qT₁ and qT₂ relaxation times and the location and volume of abnormal qT₁ and qT₂ voxels within the lesion. Uncertainties associated with onset time estimates of qT₁ and qT₂ MRI data vary from ± 25 min to ± 47 min for the first 5 hours of stroke. The most accurate onset time estimates can be obtained by quantifying the volume of overlapping abnormal qT₁ and qT₂ lesion volumes, termed 'V^overlap' (± 25 min) or by quantifying hemispheric differences in qT₂ relaxation times only (± 28 min). Overall, qT₂ derived parameters outperform those from qT₁. The current MRI protocol is tested in the hyperacute phase of a permanent focal ischemia model, which may not be applicable to transient focal brain ischemia.