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Sirtuin 7 plays an oncogenic role in human osteosarcoma via downregulating CDC4 expression

Wang Wei1,2, Zhang Xiao Jing1,2, Zheng Ke1,2

  • 1Department of Bone and Soft-Tissue Tumor Surgery, Cancer Hospital of China Medical UniversityShenyang 110042, Liaoning Province, PR China.

American Journal of Cancer Research
|October 6, 2017
PubMed

Insights

Sirtuin 7 (SIRT7) promotes osteosarcoma progression by increasing cell proliferation, migration, and invasion. Targeting SIRT7 could offer a new therapeutic strategy for osteosarcoma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • The role of Sirtuin 7 (SIRT7) in cancer remains controversial, with varying functions across different cancer types.
  • The specific involvement of SIRT7 in osteosarcoma progression is not yet understood.

Purpose of the Study:

  • To investigate SIRT7 expression levels in osteosarcoma.
  • To explore the biological functions of SIRT7 in osteosarcoma cell processes.
  • To elucidate the underlying molecular mechanisms of SIRT7 action in osteosarcoma.

Main Methods:

  • Quantitative analysis of SIRT7 expression in osteosarcoma tissues and cell lines.
  • Functional assays including proliferation, migration, invasion, tumor formation, and metastasis assays.
  • Mechanistic studies involving histone modification (H3K18ac) and gene transcription (CDC4).

Main Results:

  • SIRT7 expression is significantly elevated in osteosarcoma tissues and cell lines compared to non-tumor controls.
  • Higher SIRT7 levels correlate with a poorer prognosis in osteosarcoma patients.
  • SIRT7 knockdown inhibits osteosarcoma cell proliferation, migration, invasion, tumor formation, and metastasis.
  • SIRT7 promotes osteosarcoma progression by downregulating H3K18ac and inhibiting CDC4 transcription.
  • Silencing CDC4 partially reverses the inhibitory effects of SIRT7 knockdown.

Conclusions:

  • SIRT7 acts as an oncoprotein in osteosarcoma, promoting tumor cell proliferation, migration, and invasion.
  • SIRT7 exerts its oncogenic function by targeting CDC4 transcription via H3K18ac modification.
  • SIRT7 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
CDC4H3K18acSIRT7osteosarcoma

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