Puia dinghuensis gen. nov., sp. nov., isolated from monsoon evergreen broad-leaved forest soil

A Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, 4GSH07^T, was originally isolated from the monsoon evergreen broad-leaved forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). It grew optimally at 28-33 °C and pH 4.0. The 16S rRNA gene sequence analysis showed that strain 4GSH07^T had the highest sequence similarity of 94.0 % to Parasegetibacter terrae JCM 19942^T, and formed an independent lineage separable from other described genera of the family Chitinophagaceae. The main fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 3-OH, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and C15 : 0 2-OH. The organism contained MK-7 as the predominant respiratory quinone, and the total DNA G+C content was 50.3 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed consistently that strain 4GSH07^T represents a novel species of a novel genus of the family Chitinophagaceae, for which the name Puia dinghuensis gen. nov., sp. nov., is proposed, with 4GSH07^T (=CGMCC 1.15448^T=LMG 29214^T) as the type strain.