Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metastasis02:30

Metastasis

6.7K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.7K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

6.1K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.1K
The Tumor Microenvironment02:17

The Tumor Microenvironment

7.9K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.9K
Tumor Immunotherapy01:27

Tumor Immunotherapy

2.0K
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
2.0K
Cancer02:18

Cancer

54.8K
Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
54.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of learning curves and cross-over effects using articulating laparoscopic instruments versus standard laparoscopic instruments.

Surgical endoscopy·2026
Same author

Risk factors for posthepatectomy liver failure: a secondary analysis of prospective clinical trials.

Langenbeck's archives of surgery·2026
Same author

Tumor-infiltrating CD8<sup>+</sup>Ki-67<sup>+</sup> and CD8<sup>+</sup>LAG-3<sup>+</sup> T cells are associated with improved patient survival in retroperitoneal dedifferentiated liposarcomas and leiomyosarcomas.

Experimental and molecular pathology·2026
Same author

PIVOTS: Aligning unseen structures using preoperative to intraoperative volume-to-surface registration for liver navigation.

Medical image analysis·2026
Same author

Pancreatic exocrine insufficiency after distal pancreatectomy - an underestimated challenge.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2026
Same author

Appendix300: Surgical video and patient metadata of 330 laparoscopic appendectomy cases from five institutions.

Scientific data·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 21, 2026

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research
07:47

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Published on: December 25, 2021

4.7K

Circulating Tumor Cells.

Sebastián A García1, Jürgen Weitz2,3,4, Sebastian Schölch2,3,4

  • 1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Fetscherstr. 74, 01307, Dresden, Germany. Sebastian.Schoelch@uniklinikum-dresden.de.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2017
PubMed
Summary
This summary is machine-generated.

Distant metastases, often driven by circulating tumor cells (CTCs), limit cancer prognosis. This work details methods for isolating live CTCs from blood for further molecular study.

Keywords:
Circulating tumor cellsDistant metastasesEpCAM

More Related Videos

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications
14:14

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

16.3K
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

27.3K

Related Experiment Videos

Last Updated: Feb 21, 2026

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research
07:47

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Published on: December 25, 2021

4.7K
Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications
14:14

Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

16.3K
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

27.3K

Area of Science:

  • Oncology
  • Cell Biology
  • Cancer Metastasis Research

Background:

  • Distant metastases, not primary tumors, typically dictate patient prognosis in solid cancers.
  • Metastases originate from circulating tumor cells (CTCs) that disseminate through the bloodstream.
  • CTCs possess stem cell-like properties, including tumor-forming capacity, essential for metastasis.

Purpose of the Study:

  • To describe the enrichment and isolation of live circulating tumor cells (CTCs) from patient blood samples.
  • To enable molecular characterization and downstream applications of isolated CTCs.
  • To advance understanding of the metastatic process driven by CTCs.

Main Methods:

  • Blood sample collection from patients with solid tumors.
  • Enrichment techniques to isolate CTCs from the blood.
  • Isolation protocols for obtaining live CTCs suitable for analysis.

Main Results:

  • Successful enrichment and isolation of viable CTCs from clinical blood specimens.
  • Demonstration of CTCs' stem cell-like characteristics relevant to metastasis.
  • Establishment of a platform for downstream molecular investigations of CTCs.

Conclusions:

  • Circulating tumor cells are critical drivers of distant metastases in solid tumors.
  • The described methods allow for the isolation of live CTCs for comprehensive study.
  • This approach facilitates research into CTC biology and therapeutic strategies targeting metastasis.