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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Updated: Feb 21, 2026

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Ribosome Profiling in Maize.

Prakitchai Chotewutmontri1, Nicholas Stiffler1, Kenneth P Watkins1

  • 1Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR, 97403, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2017
PubMed
Summary
This summary is machine-generated.

This study details a robust ribosome profiling (Ribo-seq) protocol for plant leaf tissue, enabling high-resolution analysis of mRNA translation. The method efficiently captures ribosome footprints from chloroplast and cytosol, advancing plant biology research.

Keywords:
ChloroplastPlantsRibo-seqRibosome profilingTranslation

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Area of Science:

  • Molecular Biology
  • Plant Science
  • Genomics

Background:

  • Ribosome profiling (Ribo-seq) offers genome-wide, high-resolution insights into mRNA translation.
  • Its application in plant biology is emerging, with limited established protocols.

Purpose of the Study:

  • To provide a detailed and simplified protocol for ribosome profiling in plant leaf tissue.
  • To enable quantitative analysis of ribosome occupancy in plants.

Main Methods:

  • Developed and optimized a Ribo-seq protocol using maize leaf tissue.
  • Successfully applied the protocol to Arabidopsis and tobacco.
  • The method captures ribosome footprints from both chloroplast and cytosol.

Main Results:

  • The protocol is robust and simpler compared to previous plant Ribo-seq methods.
  • Successfully profiled ribosomes in maize, Arabidopsis, and tobacco.
  • Efficiently detected ribosome footprints in chloroplast and cytosol.

Conclusions:

  • This protocol facilitates ribosome profiling in diverse plant species.
  • It offers a valuable tool for investigating mRNA translation in plant biology.
  • The method is suitable for studying chloroplast and cytosolic ribosome activity.