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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Isolation and characterization of 30 microsatellite loci for Cunninghamia lanceolata (Taxodiaceae)
Si-Si Wang1, Yang Zhang1, De-Chen Liu1
1School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Premise Of The Study:
To quantify the population-level genetic characteristics of Cunninghamia lanceolata (Taxodiaceae), an important timber conifer, we developed 30 pairs of microsatellite primers based on the nuclear genome.
Methods And Results:
Using the streptavidin-biotin capture system, we developed 14 polymorphic and 16 monomorphic microsatellites. Polymorphisms were detected in 14 loci using 94 individual trees that were collected from three C. lanceolata populations in Hubei and Zhejiang provinces and in Chongqing Municipality, China. There were three to 30 alleles per locus, and the observed and expected heterozygosities ranged from 0.0313-0.8333 and from 0.0313-0.9246, respectively. Cross-species amplification showed that two to seven polymorphic loci were functional in three of the five related species that were collected.
Conclusions:
Our newly developed microsatellite primers provide neutral molecular markers that are beneficial to future studies of population genetics and germplasm conservation of C. lanceolata.
