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MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma

Lu Jin1, Yifan Li1, Liping Nie2

  • 1Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.

Molecular Medicine Reports
|October 10, 2017
PubMed

Insights

MicroRNAs (miRNAs) play a role in cancer. This study found miR-24-2 is upregulated in renal cell carcinoma (RCC) and promotes cancer cell proliferation, migration, and invasion, suggesting it may be an oncogene in RCC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Expression Regulation

Background:

  • MicroRNAs (miRNAs) are crucial regulators of cellular processes, and their dysregulation is linked to tumorigenesis.
  • Previous studies indicated miR-24 downregulation in renal cell carcinoma (RCC), with conflicting roles as an oncogene or tumor suppressor in various cancers.

Purpose of the Study:

  • To investigate the expression levels of miR-24-1 and miR-24-2 in RCC tissues and cell lines.
  • To elucidate the functional role of miR-24-2 in RCC cell proliferation, migration, invasion, and apoptosis.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to assess miR-24-1 and miR-24-2 expression.
  • miR-24-2 mimic and inhibitor transfections were performed in RCC cell lines (786-O and ACHN).
  • Cell proliferation, migration, invasion, and apoptosis assays were conducted to evaluate miR-24-2's function.

Main Results:

  • miR-24-2 was significantly upregulated in RCC tissues and cell lines (ACHN, 786-O, 769P) compared to healthy controls and HEK-293T cells.
  • miR-24-1 expression was nearly absent in both RCC and healthy kidney tissues.
  • Overexpression of miR-24-2 promoted RCC cell proliferation, migration, and invasion, while its inhibition suppressed these processes.
  • miR-24-2 was found to influence apoptosis in RCC cells.

Conclusions:

  • miR-24-2 acts as an oncogene in renal cell carcinoma, promoting key cancer hallmarks.
  • miR-24-2 warrants further investigation for its signaling pathways and potential as a therapeutic target or early detection biomarker for RCC.

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