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'Cialit' as a tissue preservative: a microbiological assessment.
B D Cookson1, P N Hoffman, T Price
1Dept. of Microbiology, United Medical School of Guys Hospital, London, UK.
The Journal of Hospital Infection
|April 1, 1988
Summary
Bacterial contamination persisted in a cartilage bank despite protocol changes over three years. Enhanced microbiological screening and alternative preservation methods are crucial for tissue banking safety.
Area of Science:
- Microbiology
- Tissue Engineering
- Orthopedics
Background:
- Cartilage banking is vital for orthopedic procedures.
- Cryopreservation using agents like 'Cialit' is a common method.
- Maintaining sterility in tissue banks is a significant challenge.
Purpose of the Study:
- To investigate the persistence of bacterial contamination in a 'Cialit'-preserved cartilage bank.
- To evaluate the effectiveness of changes in harvesting and preservation protocols.
- To highlight the need for robust microbiological screening in tissue banking.
Main Methods:
- A 3-year prospective study was conducted.
- Bacterial contamination was monitored throughout the study period.
- Modifications were made to harvesting and preservation protocols.
Main Results:
- Bacterial contamination was detected and persisted despite protocol modifications.
- The study identified ongoing issues with the sterility of preserved cartilage.
- The effectiveness of implemented changes in reducing contamination was limited.
Conclusions:
- Adequate microbiological screening is essential for tissue banks.
- Current preservation protocols may be insufficient to prevent contamination.
- Alternative tissue preservation strategies should be explored to ensure graft safety.