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Updated: Feb 21, 2026
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
Published on: August 19, 2018
Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line
Xiao Shen1,2, Danijel Dojcinovic3,4,5, Lucia Baldi6
1Laboratory of Cellular Biotechnology, Faculty of Life Sciences, École Polytechnique Fédérale de Lausanne, 1015, Lausanne, Switzerland. xiao.shen@cantonbio.com.
Objectives:
To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line.
Results:
When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recombinant major histocompatibility (MHC) class II protein (HLA-DR12xHis) increased four-fold over the levels observed for the same cells cultivated in roller bottles (RB) without vented caps. Culturing in RB with vented caps while increasing the rotation speed from 6 rpm to 18 rpm also improved cell growth five-fold and protein productivity three-fold which is comparable to the levels observed in the orbitally shaken containers. Protein activity was found to be almost identical between the two vessel systems tested.
Conclusions:
Optimized cell culture conditions and a more efficient vessel type can enhance gas transfer and mixing and lead to substantial improvement of recombinant product yields from S2 cells.
