Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enhanced surface acoustic wave cell sorting by 3D microfluidic-chip design.

W L Ung1, K Mutafopulos, P Spink

  • 1School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Lab on a Chip
|October 11, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Propagation of extended fractures by local nucleation and rapid transverse expansion of crack-front distortion.

Nature physics·2024
Same author

Classification of chemically modified red blood cells in microflow using machine learning video analysis.

Soft matter·2023
Same author

Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics.

Angewandte Chemie (International ed. in English)·2023
Same author

Dynamic Speckle Holography.

Physical review letters·2021
Same author

The vortex-driven dynamics of droplets within droplets.

Nature communications·2021
Same author

Acoustic erythrocytometer for mechanically probing cell viscoelasticity.

Lab on a chip·2020
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

This study presents a new microfluidic cell sorter using acoustic waves for high-purity sorting. The device offers microfluidic advantages with performance comparable to advanced fluorescence-activated cell sorters.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Acoustic wave devices

Background:

  • Microfluidic cell sorting offers advantages over traditional methods.
  • Existing acoustic cell sorters face limitations in actuation and control.
  • Multilayer device fabrication and planar surface acoustic wave excitation present opportunities for improvement.

Purpose of the Study:

  • To develop an acoustic wave-driven microfluidic cell sorter.
  • To enhance cell actuation and deflection using refracted acoustic waves and asymmetric flow.
  • To achieve precise 3-dimensional flow control via topographical structures.

Main Methods:

  • Utilized multilayer device fabrication with planar surface acoustic wave excitation.
  • Employed an asymmetric flow field to increase cell deflection.

Related Experiment Videos

  • Integrated topographical structures on the microchannel for 3D flow control.
  • Experimentally quantified the impact of structural dimensions and acoustic parameters.

    • Main Results:

    • Demonstrated enhanced cell actuation through the vertical component of refracted acoustic waves.
    • Achieved precise 3-dimensional flow control within the microchannel.
    • Cell sorting rates and purities approached those of state-of-the-art fluorescence-activated cell sorters.
    • Validated the effectiveness of topographical structures and acoustic parameters.

    Conclusions:

    • The developed acoustic wave-driven microfluidic cell sorter combines fabrication advantages with efficient cell actuation.
    • The device achieves high sorting performance comparable to fluorescence-activated cell sorters.
    • This technology offers a promising alternative for microfluidic cell sorting applications.