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Middle Ear Prosthesis with Bactericidal Efficacy-In Vitro Investigation.
Magdalena Ziąbka1, Michał Dziadek2, Elżbieta Menaszek3
1AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, Krakow 30-059, Poland. ziabka@agh.edu.pl.
Molecules (Basel, Switzerland)
|October 11, 2017
Summary
This study developed a new antibacterial middle ear prosthesis using ABS polymer with silver nanoparticles. The material showed no cytotoxicity and effectively eliminated bacteria, preventing infection during ossicular reconstruction.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Medical Devices
Background:
- Ossicular replacement prostheses require biocompatible materials to prevent infection like otitis media.
- Incorporating antibacterial agents is crucial for preventing illness during ossicular reconstruction.
Purpose of the Study:
- To evaluate the biological properties of a novel composite prosthesis made from acrylonitrile butadiene styrene (ABS) incorporating silver nanoparticles (AgNPs).
- To assess the antibacterial efficacy, cytotoxicity, and surface characteristics of the AgNP-containing ABS composite for potential use in middle ear prostheses.
Main Methods:
- ABS composite samples with varying concentrations of silver nanoparticles (AgNPs) were fabricated using injection molding and extrusion.
- In vitro studies assessed bactericidal activity against *Staphylococcus aureus* and *Pseudomonas aeruginosa*, cell proliferation, viability, and cytotoxicity using Hs680.Tr. fibroblast cells.
- Surface parameters (roughness, wettability) and silver ion release kinetics were analyzed.
Main Results:
- The composite materials exhibited continuous silver ion release, increasing with time and AgNP concentration.
- No cytotoxicity was observed in fibroblast cells, while significant bactericidal efficacy against tested bacteria was demonstrated.
- Samples with AgNPs showed increased surface roughness and enhanced hydrophilic properties.
- Antimicrobial efficacy was achieved even at low AgNP concentrations due to effective silver ion release.
Conclusions:
- The developed ABS composite with silver nanoparticles demonstrates excellent biocompatibility and potent antibacterial properties.
- This novel material holds significant promise for the development of effective middle ear prostheses that can prevent infections during ossicular reconstruction.
- The controlled release of silver ions contributes to the material's antimicrobial efficacy, addressing a critical need in otologic surgery.