[A modified Tanabe-Chiba medium for detection of Entamoeba histolytica]

Tanabe-Chiba's (T-C) medium, which has been routinely used for detection of Entamoeba histolytica in feces in Japan, was modified by adding some commercially available enrichments to the liquid part and by eliminating the agar slant. This is tentatively called M medium. M medium and T-C medium were compared for their efficiency for detecting E. histolytica in the feces of monkeys. No significant difference in the detection rate was found between the two media. However the rate of multiplication of the amoebae in M medium was significantly higher than that in T-C medium within the limited time of 24-48 h of cultivation.