Related Experiment Video
Updated: Feb 21, 2026
09:13
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
18.1K
WSSV-responsive gene expression under the influence of PmVRP15 suppression
Phawida Tummamunkong1, Phattarunda Jaree1, Anchalee Tassanakajon1
1Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Bangkok 10330, Thailand.
Fish & Shellfish Immunology
|October 12, 2017
Summary
The viral responsive protein 15 (PmVRP15) in shrimp influences hemocyte homeostasis during white spot syndrome virus (WSSV) infection. Silencing PmVRP15 alters immune gene expression, impacting shrimp defense mechanisms against WSSV.
Area of Science:
- Aquaculture
- Marine Biology
- Immunology
Background:
- White Spot Syndrome Virus (WSSV) poses a significant threat to shrimp aquaculture.
- Viral Responsive Protein 15 (PmVRP15) is upregulated in Penaeus monodon hemocytes during WSSV infection.
- Understanding host-pathogen interactions is crucial for disease management in shrimp.
Purpose of the Study:
- To investigate differential gene expression in shrimp hemocytes during WSSV infection influenced by PmVRP15.
- To identify immune-related genes affected by PmVRP15 knockdown in WSSV-infected shrimp.
Main Methods:
- Suppression subtractive hybridization (SSH) was employed to compare gene expression profiles.
- PmVRP15 was silenced in WSSV-infected shrimp using RNA interference (RNAi).
- Quantitative real-time PCR (qRT-PCR) was used to validate differential gene expression.
Main Results:
- SSH identified distinct sets of genes in the presence and absence of PmVRP15.
- Genes related to defense/homeostasis and energy metabolism were abundant.
- Hemocyte homeostasis-associated protein (PmHHAP) and granulin-like protein were highly upregulated when PmVRP15 was absent.
- Transglutaminase II, involved in hematopoietic regulation, was downregulated.
Conclusions:
- PmVRP15 plays a role in regulating hemocyte homeostasis during WSSV infection in Penaeus monodon.
- The absence of PmVRP15 leads to significant alterations in immune gene expression, including upregulation of PmHHAP and granulin-like protein.
- PmVRP15 may be a key factor in the shrimp's immune response to WSSV.
