Related Experiment Video

Updated: May 1, 2026

Characterization Of Multi-layered Fish Scales Atractosteus spatula Using Nanoindentation, X-ray CT, FTIR, and SEM
10:06

Characterization Of Multi-layered Fish Scales Atractosteus spatula Using Nanoindentation, X-ray CT, FTIR, and SEM

Published on: July 10, 2014

15.0K

Functional characterization of PmPen5 from the black tiger shrimp Penaeus monodon

Vichien Rimphanitchayakit1, Premruethai Supungul2, Thaweesak Songserm3

  • 1Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok, 10330, Thailand.

Fish & Shellfish Immunology
|April 29, 2026
PubMed

Insights

Penaeidin5 (PmPen5) is a vital shrimp antimicrobial peptide. This study shows PmPen5 fights viral and bacterial infections, and its expression is regulated by the Imd pathway, crucial for shrimp immunity.

Area of Science:

  • Aquatic immunology
  • Molecular biology
  • Infectious diseases

Background:

  • Penaeidin5 (PmPen5) is a key antimicrobial peptide in shrimp immunity.
  • Its role in shrimp defense against pathogens requires further investigation.

Purpose of the Study:

  • To investigate the function of Penaeus monodon Penaeidin5 (PmPen5) during pathogenic infections.
  • To elucidate the regulatory pathway controlling PmPen5 expression.
  • To assess the antimicrobial and antiviral potential of PmPen5.

Main Methods:

  • Gene expression analysis of PmPen5 in shrimp hemocytes post-infection with Yellow Head Virus (YHV), White Spot Syndrome Virus (WSSV), Vibrio harveyi, and Vibrio parahaemolyticus AHPND.
  • PmPen5 gene knockdown and subsequent analysis of immune-related gene expression and mortality rates.
  • Administration of recombinant PmPen5 to assess its therapeutic effect.
  • Analysis of PmPen5 expression in relation to PmRelish protein and the Imd pathway.
  • In vitro fungicidal activity assays against Lasiodiplodia theobromae.

Main Results:

  • PmPen5 expression was significantly upregulated in shrimp hemocytes following YHV, WSSV, V. harveyi, and V. parahaemolyticus AHPND infections.
  • Knockdown of PmPen5 exacerbated YHV infection severity and mortality, while recombinant PmPen5 administration partially restored survival.
  • PmPen5 expression correlated with PmRelish protein, indicating regulation via the immune deficiency (Imd) pathway.
  • PmPen5 demonstrated in vitro fungicidal activity against Lasiodiplodia theobromae.

Conclusions:

  • PmPen5 plays a critical role in shrimp innate immunity against viral and bacterial pathogens.
  • The Imd pathway regulates PmPen5 expression, highlighting its importance in shrimp immune response.
  • PmPen5 exhibits both antiviral and fungicidal properties, making it a significant component of shrimp defense mechanisms.
Keywords:
Antimicrobial proteinsPenaeus monodonPmPen5Vibrio harveyiVibrio parahaemolyticus AHPNDYellow head virus

More Related Videos

The Swimmeret System of Crayfish: A Practical Guide for the Dissection of the Nerve Cord and Extracellular Recordings of the Motor Pattern11:45

The Swimmeret System of Crayfish: A Practical Guide for the Dissection of the Nerve Cord and Extracellular Recordings of the Motor Pattern

Published on: November 25, 2014

12.1K
Assaying Predatory Feeding Behaviors in Pristionchus and Other Nematodes06:27

Assaying Predatory Feeding Behaviors in Pristionchus and Other Nematodes

Published on: September 4, 2016

10.7K

Related Concept Videos

ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
5.1K
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.0K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.8K
Fixed Action Patterns01:06

Fixed Action Patterns

A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
16.0K