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Related Experiment Video

Updated: Feb 21, 2026

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues

Published on: May 1, 2019

DIGE Analysis of Human Tissues.

Cecilia Gelfi^1,2,3, Daniele Capitanio⁴

¹Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, via f.lli Cervi, 93, 20090, Segrate, Milan, Italy. cecilia.gelfi@unimi.it.

Methods in Molecular Biology (Clifton, N.J.)

|October 12, 2017

Summary

This summary is machine-generated.

Two-dimensional difference gel electrophoresis (2-D DIGE) offers precise comparative protein analysis. This advanced technique uses fluorescent labeling and statistical analysis for accurate, validated results in proteomics research.

Keywords:

2-D DIGE CyDye DIGE fluors Differential analysis Two-dimensional electrophoresis

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Last Updated: Feb 21, 2026

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High-Throughput, Multi-Image Cryohistology of Mineralized Tissues

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Published on: September 14, 2016

Area of Science:

Proteomics
Biochemistry
Analytical Chemistry

Background:

Comparative protein assessment is crucial in biological research.
Existing methods may lack accuracy or throughput.
Advanced electrophoretic techniques are needed for reliable protein quantification.

Purpose of the Study:

To introduce and describe the principles of two-dimensional difference gel electrophoresis (2-D DIGE).
To highlight 2-D DIGE as an accurate quantitative method for comparative protein analysis.
To emphasize the robustness and validation potential of 2-D DIGE data.

Main Methods:

Utilizes two-dimensional gel electrophoresis (2-DE) for protein separation.
Employs fluorescent labeling of protein extracts to avoid altering chemical properties.
Incorporates two-dye or three-dye systems and an internal pooled standard for quantitative accuracy.

Main Results:

2-D DIGE enables separation of multiple protein samples on a single gel.
Image matching and cross-gel statistical analysis yield robust quantitative results.
Data generated by 2-D DIGE is amenable to validation by independent technologies.

Conclusions:

2-D DIGE is an advanced and accurate tool for comparative protein analysis.
The method ensures high precision and reliability in quantitative proteomics.
Its robust data facilitates successful validation, advancing biological research.