Related Experiment Video
Updated: Feb 21, 2026
07:54
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Published on: August 29, 2018
9.1K
Rapid 2D DIGE Proteomic Analysis of Mouse Liver
1Laboratory of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo, 194-8543, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2017
Summary
Two-dimensional difference gel electrophoresis (2D DIGE) provides rapid, direct visualization and quantification of thousands of proteins. This proteomic analysis method is crucial for identifying differentially expressed proteins in mouse liver under pathological conditions.
Area of Science:
- Proteomics
- Biochemistry
- Animal Models
Background:
- Liquid chromatography-mass spectrometry (LC-MS/MS) is the current standard for proteomic analysis.
- Conventional two-dimensional difference gel electrophoresis (2D DIGE) remains a valuable technique for protein visualization and quantification.
Purpose of the Study:
- To describe a routine 2D DIGE proteomic analysis workflow.
- To analyze protein expression in mouse liver under pathological conditions.
Main Methods:
- Utilized 2D DIGE for global proteomic views within 3 days.
- Employed MALDI-TOF/MS and MASCOT for protein identification within a week.
- Focused on liver tissue isolated from mice with pathological conditions.
Main Results:
- Achieved rapid global proteomic views using 2D DIGE.
- Successfully identified proteins exhibiting differential expression levels.
- Demonstrated the utility of 2D DIGE in pathological mouse liver studies.
Conclusions:
- 2D DIGE is an efficient method for comprehensive proteomic analysis.
- This technique facilitates the identification of key proteins in disease states.
- The described workflow is suitable for routine proteomic investigations in animal models.
