Fluorescent chemosensors: the past, present and future
Di Wu1, Adam C Sedgwick2, Thorfinnur Gunnlaugsson3
1Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea. jyoon@ewha.ac.kr.
Chemical Society Reviews
|October 12, 2017
Summary
This review explores fluorescent chemosensors, highlighting their 150-year history and applications in biology and environmental science. It discusses design principles, challenges, and future directions for these vital detection tools.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Environmental Science
Background:
- Fluorescent chemosensors have a 150-year history.
- They are widely used in biology, physiology, pharmacology, and environmental sciences.
- Significant progress has been made, yet challenges remain.
Purpose of the Study:
- To provide a historical overview of fluorescent sensor research.
- To highlight key contributions from global research groups.
- To discuss design principles, challenges, and future directions.
Main Methods:
- Review of pioneering and representative works.
- Analysis of fundamental principles in chemosensor design.
- Discussion of current challenges and future research avenues.
Main Results:
- A comprehensive overview of fluorescent chemosensor development.
- Identification of key advancements and influential research.
- Exploration of applications in established and emerging biotechnologies.
Conclusions:
- Fluorescent chemosensors are crucial for detecting various species.
- The field has a rich history and continues to evolve.
- Future applications in biotechnology are promising.
Related Concept Videos
01:14Photoluminescence: Applications
1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K
01:26Variables Affecting Phosphorescence and Fluorescence
1.6K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
1.6K
02:07FISH - Fluorescent In-situ Hybridization
24.8K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
24.8K
01:25Fluorescence and Phosphorescence: Instrumentation
1.6K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.6K
02:11Reporter Genes
13.5K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.5K
01:23Photoluminescence: Fluorescence and Phosphorescence
4.0K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
A pair of electrons in a...
4.0K