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BiodentineTM is cytocompatible with human primary osteoblasts.

Miriam Zaccaro Scelza1, Joyce Costa Nascimento2, Licinio Esmeraldo da Silva3

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Biodentine (BD) and Mineral Trioxide Aggregate (MTA) showed good initial cell viability. However, long-term exposure to MTA extracts proved cytotoxic, while Biodentine remained cytocompatible with human osteoblasts.

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Area of Science:

  • Biomaterials Science
  • Dental Materials
  • Cell Biology

Background:

  • Calcium silicate-based materials, like Biodentine (BD), are investigated as alternatives to Mineral Trioxide Aggregate (MTA).
  • BD is designed for dentin replacement in various endodontic applications.
  • Assessing osteoblast response is crucial for evaluating material biocompatibility in bone contact.

Purpose of the Study:

  • To evaluate the in vitro cytocompatibility of Biodentine (BD) with human primary osteoblasts.
  • To compare BD's cellular response against Mineral Trioxide Aggregate (MTA) using osteoblasts.
  • To analyze cell viability through mitochondrial activity, membrane integrity, and cell density.

Main Methods:

  • Biodentine (BD) and MTA extracts were prepared after 24-hour and 42-day incubation periods.
  • Primary human osteoblasts were exposed to these extracts.
  • Cell viability was assessed using XTT, NRU, and CVDE assays.

Main Results:

  • Both BD and MTA extracts showed >70% cell viability after 24-hour extraction.
  • MTA extracts exhibited significant cytotoxicity (p < 0.05) compared to control after extended conditioning.
  • BD showed a significant reduction in cell viability after 42 days (p<0.05) but remained above the 70% biocompatibility threshold.

Conclusions:

  • Biodentine (BD) demonstrates cytocompatibility with human primary osteoblasts.
  • BD is suitable for direct contact with bone tissues in endodontic applications.
  • MTA may exhibit cytotoxic effects with prolonged material conditioning.