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Updated: Aug 6, 2026
Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
Published on: December 21, 2014
Biological Activity Alterations of Human Amniotic Membrane Pre and Post Irradiation Tissue Banking
Waleed Nemr1, A S Bashandy1, Eman Araby1
1Department of Radiation Microbiology, National Centre for Radiat ion Research and Technology (NCRRT), Atomic Energy Authority (AEA),P.O. Box 29, Nasr City, Cairo, Egypt.
Background And Objective:
Innate immunity of Human Amniotic Membrane (HAM) and its highly active secretome that rich with various types of growth factors and anti-inflammatory substances proposed it as a promising material for many medical studies and applications.
Methodology:
This study evaluate the biological activity of cultivated HAM pre and post tissue banking process in which freeze-dried HAM was sterilized by 25 KGray (kGy) dose of γ radiation. The HAM's antimicrobial activity, viability, growth of isolated human amniotic epithelial cells (HAECs), hematopoietic stimulation of co-cultivated murine bone marrow cells (mammalian model), scaffold efficiency for fish brain building up (non-mammalian model) and self re-epithelialization after trypsin denuding treatment were examined as supposed biological activity features.
Results:
Native HAM revealed viability indications and was active to kill all tested microorganisms; 6 bacterial species (3 Gram-positive and 3 Gram-negative) and Candida albicans as a pathogenic fungus. Also, HAM activity promoted colony formation of murine hematopoietic cells, Tilapia nilotica brain fragment building-up and self re-epithelialization after trypsin treatment. In contrary, radiation-based tissue banking of HAM caused HAM cellular death and consequently lacked almost all of examined biological activity features.
Conclusion:
Viable HAM was featured with biological activity than fixed HAM prepared by irradiation tissue banking.
