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High expression of Sam68 in sacral chordomas is associated with worse clinical outcomes
Hai Wen1, Pengzhi Li1, Hong Ma1
1Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Abstract:
Src-associated in mitosis of 68 kDa (Sam68), also known as KHDRBS1 (KH domain-containing, RNA-binding, signal transduction-associated 1), is a member of the signal transduction and activation of RNA family. Previous studies have demonstrated that the aberrant expression of Sam68 is associated with the progression and prognosis of a variety of cancers, but little is known about its expression and role in chordomas, which are rare and aggressive bone neoplasms. In this study, we analyzed 40 tumor tissues and 20 distant normal tissues obtained from 40 patients with sacral chordoma using immunohistochemistry, and observed the expression of Sam68 was significantly upregulated in sacral chordomas compared with normal tissues (P=0.001). A positive correlation between the expression of Sam68 and the cell proliferation index Ki-67 was determined using Spearman's rank correlation test (γ =0.599, P<0.001). In addition, high expression of Sam68 was significantly associated with surrounding muscle invasion (P<0.001). Moreover, Kaplan-Meier curves showed that patients with overexpressed Sam68 had shorter local recurrence-free survival time (P<0.001). Lastly, multivariate analysis indicated that Sam68 is an independent prognostic factor for the local recurrence-free survival of sacral chordomas (hazard ratio =5.929, 95% CI: 1.092-32.188, P=0.039). Our findings suggest the use of Sam68 as a predictor for the recurrence of sacral chordomas.
Insights
Src-associated in mitosis of 68 kDa (Sam68) is upregulated in sacral chordomas, correlating with proliferation and invasion. Overexpressed Sam68 predicts shorter recurrence-free survival, suggesting its use as a prognostic marker.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Src-associated in mitosis of 68 kDa (Sam68), also known as KHDRBS1, is implicated in cancer progression.
- The role and expression of Sam68 in chordomas, particularly sacral chordomas, remain largely uncharacterized.
Purpose of the Study:
- To investigate the expression and prognostic significance of Sam68 in sacral chordomas.
- To determine the correlation between Sam68 expression and clinicopathological features, including proliferation and local recurrence.
Main Methods:
- Immunohistochemistry was used to analyze Sam68 expression in 40 sacral chordoma tissues and 20 normal tissues.
- Spearman's rank correlation and Kaplan-Meier analysis were employed to assess correlations and survival outcomes.
- Multivariate analysis identified independent prognostic factors.
Main Results:
- Sam68 expression was significantly upregulated in sacral chordomas compared to normal tissues (P=0.001).
- Sam68 expression positively correlated with the proliferation index Ki-67 (γ=0.599, P<0.001) and associated with surrounding muscle invasion (P<0.001).
- High Sam68 expression predicted shorter local recurrence-free survival (P<0.001) and was an independent prognostic factor (HR=5.929, P=0.039).
Conclusions:
- Sam68 is significantly overexpressed in sacral chordomas.
- Sam68 expression is linked to tumor proliferation, invasion, and poorer prognosis.
- Sam68 serves as a potential predictor for the recurrence of sacral chordomas.