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Updated: Feb 20, 2026
Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
Published on: November 21, 2012
Dual-Signal Microbial Biosensor for the Detection of Dopamine without Inference from Other Catecholamine
1Department of Chemistry, National Taiwan Normal University , 88, Section 4, Tingzhou Road, Taipei 11677, Taiwan.
Abstract:
Dopamine, one of catecholamine neurotransmitters, plays an important role in many brain functions and behavioral responses. In this study, we developed a novel dual-signal whole-cell biosensor for the detection of dopamine through the generation of red fluorescent proteins and 6-decarboxylated betaxanthin pigments. The proposed system responses specifically to dopamine with a detection limit of 1.43 μM. Furthermore, a combination of dual output signals makes it possible to reduce the interference from other catecholamine neurotransmitters, including L-DOPA, epinephrine, and norepinephrine.
