Beyond the bulk: disclosing the life of single microbial cells

Katrin Rosenthal^1,2, Verena Oehling^1,2, Christian Dusny¹

¹Department Solar Materials, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany.

|October 14, 2017

Abstract:

Microbial single cell analysis has led to discoveries that are beyond what can be resolved with population-based studies. It provides a pristine view of the mechanisms that organize cellular physiology, unbiased by population heterogeneity or uncontrollable environmental impacts. A holistic description of cellular functions at the single cell level requires analytical concepts beyond the miniaturization of existing technologies, defined but uncontrolled by the biological system itself. This review provides an overview of the latest advances in single cell technologies and demonstrates their potential. Opportunities and limitations of single cell microbiology are discussed using selected application-related examples.

Keywords:

heterogeneity lab-on-a-chip microfluidics single cell analysis single cell dynamics technical bias

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Abstract:

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