Related Experiment Video
Updated: Jul 28, 2026
07:03
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
Published on: January 2, 2018
An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts.
Mithun Mitra1,2, Linda D Ho1, Hilary A Coller3,4
1Department of Molecular, Cell and Developmental Biology, 5145 Terasaki Life Science Building, 610 Charles E. Young Drive E., University of California, Los Angeles, 90095-7329, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2017
Summary
This study presents an in vitro model using human dermal fibroblasts to explore cellular quiescence. Researchers detail methods for inducing and manipulating this reversible cell cycle exit to identify key regulatory factors.
Area of Science:
- Cell Biology
- Developmental Biology
Background:
- Cellular quiescence is a reversible cell cycle exit crucial for stress adaptation and tissue homeostasis.
- Understanding quiescence is vital for normal development, wound healing, and diseases like cancer.
- In vitro models are essential for controlled study of quiescence mechanisms.
Purpose of the Study:
- To establish and characterize an in vitro model of cellular quiescence.
- To investigate the induction, maintenance, and exit from quiescence.
- To identify critical regulators of the quiescent state in human fibroblasts.
Main Methods:
- Isolation of neonatal human dermal fibroblasts from skin.
- Induction of quiescence via antiproliferative signals, contact inhibition, and serum starvation (mitogen withdrawal).
- Manipulation of fibroblasts in both proliferating and quiescent states for regulatory analysis.
Main Results:
- Successfully established an in vitro model of quiescence using human dermal fibroblasts.
- Demonstrated methods to induce and maintain fibroblasts in a quiescent state.
- Developed approaches to study factors regulating quiescence.
Conclusions:
- The described in vitro model provides a robust platform for studying cellular quiescence.
- This model facilitates the identification of critical regulators governing entry, exit, and maintenance of quiescence.
- Further research using this model can elucidate the role of quiescence in development and disease.
