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Related Experiment Videos

Cell Cycle Analysis by Mass Cytometry.

Gregory K Behbehani1

  • 1Division of Hematology, Ohio State University and James Cancer Hospital, Starling Loving Hall, 320 W. 10th Avenue, Columbus, OH, 43210, USA. Gregory.behbehani@osumc.edu.

Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2017
PubMed
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Mass cytometry enables detailed cell cycle analysis alongside other cellular markers. This method accurately identifies all five cell cycle phases in various sample types, advancing cancer and immunology research.

Area of Science:

  • Cellular Biology
  • Immunology
  • Oncology

Background:

  • Cell cycle progression is crucial for tumor development, therapy response, immune regulation, and development.
  • Flow cytometry is established for cell cycle analysis, but mass cytometry offers multi-parametric analysis.
  • Mass cytometry allows simultaneous measurement of cell cycle and up to 39 other antigens.

Purpose of the Study:

  • To describe a mass cytometry method for analyzing cell cycle state.
  • To enable characterization of interactions between cell cycle and cellular processes.
  • To apply the method to diverse biological samples.

Main Methods:

  • Utilized mass cytometry for cell cycle analysis.
  • Incorporated 5-Iodo-2'-deoxyuridine (IdU) labeling.
Keywords:
Cell cycleCyTOFCyclinIodo-deoxyuridineKi-67Mass cytometryPhosphorylated histone H3Retinoblastoma protein

Related Experiment Videos

  • Measured phosphorylated retinoblastoma protein (pRb), cyclin B1, and phosphorylated histone H3 (p-HH3).
  • Applied to in vitro cell lines, ex vivo human blood/bone marrow, and in vivo murine tissues.

    • Main Results:

    • Developed a robust gating strategy for cell cycle phase separation.
    • Successfully analyzed cell cycle in multiple sample types.
    • Integrated cell cycle markers with up to 39 other cellular antigens.

    Conclusions:

    • Mass cytometry provides a powerful tool for comprehensive cell cycle analysis.
    • This method enhances understanding of cell cycle's role in various biological contexts.
    • Facilitates deeper insights into cancer, immunity, and development.