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Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells
Marla M Spain1, Sarah G Swygert1, Toshio Tsukiyama2
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Mail Stop A1-162, P.O. Box 19024, Seattle, WA, 98109-1024, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2017
Abstract:
Saccharomyces cerevisiae enter quiescence during extended growth in culture (greater than 7 days). Here, we describe a method to separate quiescent from non-quiescent cells by density gradient. We also describe approaches for DAPI staining the chromatin of quiescent cells, measuring quiescent cell viability, and extracting RNA from quiescent cells for use in genomics experiments.