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Related Experiment Videos

Structure function characterization of the ELL Associated Factor (EAF) from Schizosaccharomyces pombe.

Preeti Dabas1, Kumari Sweta1, Mary Ekka2

  • 1University School of Biotechnology, G.G.S. Indraprastha University, Sector16C, Dwarka, New Delhi 110078, India.

Gene
|October 17, 2017
PubMed
Summary

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ELL Associated Factor (EAF) protein in S. pombe regulates cell growth and DNA damage survival. Its disordered carboxyl-terminus is crucial for function, transitioning to an alpha-helical state to rescue growth defects.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • ELL Associated Factor (EAF) proteins enhance transcription elongation by interacting with ELL.
  • The function of EAF homolog in Schizosaccharomyces pombe (SpEAF) is not well understood.
  • Unlike essential higher eukaryotic homologs, SpEAF is not vital for S. pombe survival.

Purpose of the Study:

  • Investigate the physiological role of SpEAF in S. pombe.
  • Determine the structure-function relationships of SpEAF domains.
  • Elucidate the role of SpEAF in cellular responses to normal growth, DNA damage, and environmental stress.

Main Methods:

  • In vivo structure-function studies in S. pombe.
  • Bioinformatics analysis.
  • Circular dichroism spectroscopy.
Keywords:
EAFEukaryotic transcriptionRNA polymerase IIS. pombe

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Main Results:

  • SpEAF regulates S. pombe cell growth under normal conditions and is essential for survival during DNA damage.
  • The amino-terminal domain of SpEAF interacts with ELL, while both amino and carboxyl termini can activate transcription.
  • The carboxyl-terminus, with a disordered conformation, is required for rescuing growth defects under normal and DNA damaging conditions.

Conclusions:

  • SpEAF possesses distinct structural and functional domains critical for transcriptional regulation.
  • The carboxyl-terminus's conformational flexibility plays a key role in SpEAF's function.
  • These findings offer insights into EAF protein mechanisms in controlling gene expression.