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Surface roughness and bacterial adhesion on root dentin treated with diode laser and conventional desensitizing
Maiza S Cury1, Camilla B Silva2, Ruchele D Nogueira2
1Department of Clinical Dentistry, School of Dentistry, Uberaba University, Av. Nene Sabino, 1801 Sala 2D04, Uberaba, MG, 38 055 500, Brazil.
Lasers in Medical Science
|October 17, 2017
Summary
Treatments for dentin hypersensitivity (DH) can alter root dentin surface roughness, potentially increasing root caries risk. Brushing with desensitizing toothpaste significantly increased surface roughness and bacterial adhesion, unlike diode laser or fluoride varnish treatments.
Area of Science:
- Dental Biomaterials
- Microbiology
- Surface Science
Background:
- Treatments for dentin hypersensitivity (DH) can modify root dentin surface roughness.
- Increased surface roughness may promote biofilm accumulation, elevating the risk of root caries.
- Understanding the impact of DH treatments on root surface characteristics and bacterial adhesion is crucial for preventing secondary caries.
Purpose of the Study:
- To compare the surface roughness of root dentin following various DH treatments.
- To evaluate the subsequent biofilm formation on these treated root dentin surfaces.
- To determine the relationship between surface roughness changes and bacterial adhesion after DH treatments.
Main Methods:
- Surface roughness (Sa) of bovine root dentin fragments was measured before and after treatments.
- Treatments included no treatment, 5% sodium fluoride varnish, professional desensitizing dentifrice application, toothbrushing with desensitizing dentifrice, and diode laser application.
- Following treatment, samples were incubated with Streptococcus mutans, and bacterial adhesion was quantified by counting colony-forming units (CFU).
Main Results:
- Toothbrushing with a desensitizing dentifrice resulted in the highest surface roughness variation (30.02%).
- Sodium fluoride varnish and diode laser treatments showed minimal changes in surface roughness, comparable to the control group.
- Higher bacterial adhesion (CFU) was observed on surfaces with increased roughness, particularly after toothbrushing with desensitizing dentifrice.
Conclusions:
- Conventional treatments for DH, especially toothbrushing with desensitizing dentifrice, can significantly alter root dentin surface roughness.
- Increased surface roughness correlates with greater bacterial adhesion.
- Diode laser and sodium fluoride varnish appear to be less disruptive to root dentin surface integrity concerning bacterial adhesion compared to aggressive brushing.