Related Experiment Videos
Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression
Philip P Adams1, Mollie W Jewett2
1Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 6900 Lake Nona Blvd., Orlando, FL, 32827, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 17, 2017
Abstract:
In vivo expression technology (IVET) has been applied to a variety of organisms to identify active promoters in specific environments or growth conditions of interest. Here, we describe modifications to employ this genome-wide screening method for Borrelia burgdorferi, the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the B. burgdorferi transcriptome during infection, despite the low bacterial numbers in the mammalian host environment.